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HPLC method for the determination of amineptine and its main metabolite in human plasma
Summary
Type:
HPLC
Application #:
103890
Catalog#:
720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection:
UV 220nm
Conditions:
aqueous phase containing 1.2g/l heptanesulphonate adjusted to pH 3.0 with H3PO4 - acetonitrile (62:38, v/v)
Authors:
Nicot G
Source:
J Chromatogr 306 (1984) 279 - 290
Details
Legend: Chromatograms of extracts from a) blank plasma, b) plasma spiked with 250ng/ml metabolite (1), 250ng/ml amineptine (2), and 500ng/ml internal standard (3), c) a patient plasma sample obtained 45min
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